No, but ensuring that the wheels are inline will provide a base point to make further investigations.

We're they ever inline ?

If your using the same hub and spacers, the only thing that has changed is the dish of the wheel.

Front to rear alignment is meaningless unless you have the pre rebuild dimensions to compare.

Its the same hub and spacers. Only problem is that I started to strip the bike as soon as I bought it. Never rode it as I was on crutches when I bought it. So I never had the opportunity to see what position the cams were in. I'm only questiong the wheel due to the issues i had with the front wheel.